杭州浩克生物
XRCC5
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AIF兔多克隆抗体
| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| KAOKEBIO | HK210006L | AIF兔多克隆抗体 | 200ul | 支 | 2800 |
Applications
Tested Applications:
IF, IHC, WB,ELISA
Species Specificity:
Human
Positive WB detected in:
LNCaP cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH-3T3 cells, MDCK cells, Pig brain cells
Positive IHC detected in:
human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells, human kidney tissue
Recommended dilution:
WB : 1:5000-1:50000
IHC : 1:1000-1:4000
IF : 1:200-1:800
Product Information
Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20oC storage.
Store at -20°C. Aliquoting is unnecessary for -20oC storage.
Immunogen Information
Full name:
apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight:
609 aa, 66 kDa
Observed molecular weight:
67 kDa, 57 kDa
Gene symbol
AIFM1
Synonyms
AIF, AIFM1, PDCD8
Background
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
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