Tested Applications: FC, IF, IHC, IP, WB, ELISA Cited Applications: IF, IHC, WB Species Specifi...查看全文
FC, IF, IHC, IP, WB,ELISA
IF, IHC, WB
Positive WB detected in:
A431 cells, HEK-293 cells, HeLa cells, mouse skin tissue
Positive IP detected in:
Positive IHC detected in:
human cervical cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Positive FC detected in:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Antigen affinity purification
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Calculated molecular weight:
Observed molecular weight:
39.1, CK 17, Cytokeratin 17, K17, keratin 17, KRT17, PCHC1
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.