2021/11/12一抗 910

品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKI0961A	LEF-1兔单克隆抗体	100ul	支	4688

Applications

Tested Applications:

IP, WB,ELISA

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Cited Applications:

IF, IHC, WB

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Species Specificity:

human, mouse, rat

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Cited Species:

human, mouse

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Positive WB detected in:

NCCIT cell, COLO 320 cells, Jurkat cells, SW 1990 cells

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Positive IP detected in:

SW 1990 cells

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Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Product Information

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Source:

Rabbit

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Purification method:

Antigen affinity purification

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Isotype:

IgG

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

lymphoid enhancer-binding factor 1

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Calculated molecular weight:

37 kDa

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Observed molecular weight:

40-45 kDa

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Gene symbol

LEF1

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Synonyms

LEF 1, LEF1, TCF1 alpha, TCF1ALPHA

Background

Lymphoid enhancer-binding factor 1(LEF1) belongs to a family of regulatory protein share homology with high mobility group protein-1, and it’s a nuclear protein exprssed in pre-B and T cells. LEF1 has a role in the Wnt signaling pathway and hair cell differentiation and follicle morphogenesis. LEF1 exists as seven isoforms and we detects three isoforms with MW 44 kDa, 36 kDa and 23 kDa. Together with CTNNB1 and EP300, LEF1 activates transcription of target genes. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established lasting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promoted transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivopromoted self-limited airway regeneration in the absence of injury. Thus, modulating the Lef-1 transcriptional program in MEC-derived progenitors may have regenerative medicine applications for lung diseases. (https://doi.org/10.1016/j.stem.2018.03.017)The phosphorylation may affects LEF1 protein’s theoretical molecular weight when tested.40-60 kD bands have also been reported (PMID:22261717;17063141 ).

Protocols

PRODUCT