品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKI0671B	PD-1兔单克隆抗体	1ml	支	10500

Applications

Tested Applications:

FC, IF, IHC, WB,ELISA

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Cited Applications:

IF, IHC, WB

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Species Specificity:

human, rat, mouse

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Cited Species:

human, mouse, rat

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Positive WB detected in:

mouse thymus tissue, human lymph node tissue, rat spleen tissue

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Positive IHC detected in:

human tonsillitis tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

human tonsillitis tissue

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Positive FC detected in:

MOLT-4 cells

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Recommended dilution:

WB : 1:2000-1:10000

IHC : 1:2000-1:8000

IF : 1:200-1:800

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG2b

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

programmed cell death 1

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Calculated molecular weight:

288 aa, 32 kDa

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Observed molecular weight:

32 kDa, 47-55 kDa

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Gene symbol

PDCD1

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Synonyms

CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

Background

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).