品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKI0291B	GranzymeB兔单克隆抗体	1ml	支	10500

Applications

Tested Applications:

FC, IF, IHC, WB,ELISA

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Cited Applications:

IF, IHC, WB

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Species Specificity:

human

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Cited Species:

human, mouse

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Positive WB detected in:

HL-60 cells

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Positive IHC detected in:

human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

HeLa cells

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Positive FC detected in:

HeLa cells

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Recommended dilution:

WB : 1:500-1:2000

IHC : 1:50-1:500

IF : 1:50-1:500

Product Information

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Source:

Rabbit

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Purification method:

Antigen affinity purification

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Isotype:

IgG

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

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Calculated molecular weight:

247 aa, 28 kDa

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Observed molecular weight:

33 kDa

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Gene symbol

GZMB

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Synonyms

CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, Granzyme B, GZMB, HLP, SECT

Background

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and g high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751).