品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HK510045M	CD146/MCAM鼠单克隆抗体	100ul	支	1960

Applications

Tested Applications:

FC, IHC, WB,ELISA

Species Specificity:

human

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Positive WB detected in:

HepG2 cells, HeLa cells, A375 cells, L02 cells, HUVEC cells, human placenta tissue

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Positive IHC detected in:

human liver cancer tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive FC detected in:

HeLa cells

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Recommended dilution:

WB : 1:2000-1:20000

IHC : 1:1000-1:4000

Product Information

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Source:

Mouse

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Purification method:

Protein G purification

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Isotype:

IgG1

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

melanoma cell adhesion molecule

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Calculated molecular weight:

646 aa, 72 kDa

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Observed molecular weight:

120 kDa

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Gene symbol

MCAM

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Synonyms

CD146, MCAM, MUC18

Background

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.