品牌	商品编码	商品名称	规格	单位	单价
HAOKEBIO	HK510044L	CD13鼠单克隆抗体	200ul	支	2800

Applications

Tested Applications:

FC, IF, IHC, WB,ELISA

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Cited Applications:

IF, IHC, WB

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Species Specificity:

human

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Cited Species:

human

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Positive WB detected in:

U-937 cells

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Positive IHC detected in:

human tonsillitis tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

human kidney tissue

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Positive FC detected in:

U-937 cells

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Recommended dilution:

WB : 1:3000-1:8000

IHC : 1:5000-1:20000

IF : 1:50-1:500

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG2a

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

alanyl (membrane) aminopeptidase

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Calculated molecular weight:

110 kDa

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Observed molecular weight:

150 kDa

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Gene symbol

ANPEP

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Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

Background

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M-1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)