品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HaoKebioHK510021L	BCL2鼠单克隆抗体	200ul	支	2800

Applications

Tested Applications:

FC, IF, IHC, IP, WB,ELISA

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Cited Applications:

CoIP, IF, IHC, WB

Species Specificity:

human, pig

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Cited Species:

human, pig, rat

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Positive WB detected in:

U2OS cells, U-937 cells, Jurkat cells, pig spleen tissue, THP-1 cells, HepG2 cells, HEK-293 cells, HT-1080 cells, MCF-7 cells, Raji cells

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Positive IP detected in:

U-937 cells

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Positive IHC detected in:

human appendicitis tissue, human tonsillitis tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

MCF-7 cells, human tonsillitis tissue

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Positive FC detected in:

human peripheral blood lymphocytes, Jurkat cells

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Recommended dilution:

WB : 1:2000-1:10000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:6400-1:25600

IF : 1:50-1:500

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG2b

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

B-cell CLL/lymphoma 2

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Calculated molecular weight:

26 kDa

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Observed molecular weight:

26 kDa

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Gene symbol

BCL2

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Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2

Background

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).