BCL2鼠单克隆抗体

一抗 产品 抗体 3660
BCL2鼠单克隆抗体

BCL2鼠单克隆抗体

  • 价格¥2800
  • 规格200ul
  • 货号HK510021L
  • 单位
  • 品牌HaoKebio

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HaoKebioHaoKebioHK510021LBCL2鼠单克隆抗体200ul2800

Applications

Tested Applications:

FC, IF, IHC, IP, WB,ELISA


Cited Applications:

CoIP, IF, IHC, WB

Species Specificity:

human, pig


Cited Species:

human, pig, rat


Positive WB detected in:

U2OS cells, U-937 cells, Jurkat cells, pig spleen tissue, THP-1 cells, HepG2 cells, HEK-293 cells, HT-1080 cells, MCF-7 cells, Raji cells


Positive IP detected in:

U-937 cells


Positive IHC detected in:

human appendicitis tissue, human tonsillitis tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:

MCF-7 cells, human tonsillitis tissue


Positive FC detected in:

human peripheral blood lymphocytes, Jurkat cells


Recommended dilution:

WB : 1:2000-1:10000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:6400-1:25600

IF : 1:50-1:500

Product Information


Source:

Mouse


Purification method:

Protein A purification


Isotype:

IgG2b


Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:

B-cell CLL/lymphoma 2


Calculated molecular weight:

26 kDa


Observed molecular weight:

26 kDa


Gene symbol

BCL2


Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2

Background

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

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