MHC Ⅱ(DR α chain) Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150192 Clone# BP6170 Predicted Molecular Wt: 29kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P01903 Background: Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling. Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome. Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens. In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide near N-terminal residues in Human MHC Ⅱ(DR α chain) was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Temponi, M. et al. J Immunol Methods 161 (1993) . 2. Ting, J.P. and Trowsdale, J. Cell 109 Suppl (2002) . Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colorectal carcinoma tissue labelling DR α chain of MHC Ⅱ with BP6170. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
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