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IF, IHC, WB,ELISA
Positive WB detected in:
DC2.4 cells, Raji cells, Ramos cells, Daudi cells
Positive IHC detected in:
human tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human tonsillitis tissue
WB : 1:2000-1:8000
IHC : 1:50-1:500
IF : 1:50-1:500
Protein G purification
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight:
321 aa, 36 kDa
Observed molecular weight:
BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.