品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HK510133M	OCT4鼠单克隆抗体	100ul	支	1960

Applications

Tested Applications:

IHC, WB,ELISA

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Cited Applications:

IHC, WB

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Species Specificity:

human, mouse, rat

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Cited Species:

human, mouse

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Positive WB detected in:

HEK-293 cells, NCCIT cells, Neuro-2a cells, HT-29 cells, human brain tissue

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Positive IHC detected in:

human breast cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Recommended dilution:

WB : 1:5000-1:20000

IHC : 1:250-1:1000

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG1

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

POU class 5 homeobox 1

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Calculated molecular weight:

39 kDa

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Observed molecular weight:

50-52 kDa

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Gene symbol

POU5F1

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Synonyms

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

Background

Expression of the OCT4 transcription factor is associated with an undifferentiated phenotype of human and mouse embryonic stem cells and germ cells. OCT4 (Octamer-4) is a homeodomain transcription factor of the POU family (POU5F1) involved in regulation of pluripotency during normal development and is detectable in embryonic stem (ES) and germ cells. Its expression is associated with an undifferentiated phenotype and tumors. In fact, gene knockdown of OCT4 promotes differentiation, thereby demonstrating a role for this factor in human ES cell self-renewal. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. OCT4 is one of the four key transcription factors (OCT4, SOX2, c-MYC, and KLF4) used to reprogram mouse and human fibroblasts to a pluripotent state as well as generate patient-specific iPS cells . The calculated molecular weight of OCT4 is 38 kDa, but the modified OCT4 protein is about 50 kDa.