GADD34兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210150L GADD34兔多克隆抗体 200ul 2800
HAOKEBIO HK210150M GADD34兔多克隆抗体 100ul 1960

Applications

Tested Applications:
IF, IHC, WB, ELISA

Cited Applications:
IF, IHC, IP, WB

Species Specificity:
human, mouse

Cited Species:
hamster, human, monkey, mouse, pig, rat

Positive WB detected in:
K-562 cells, HepG2 cells, Tunicamycin treated HeLa cells, Jurkat cells, PC-3 cells

Positive IHC detected in:

human pancreas tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
PC-3 cells

Recommended dilution:
WB : 1:300-1:1000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
protein phosphatase 1, regulatory (inhibitor) subunit 15A

Calculated molecular weight:
73 kDa

Observed molecular weight:
100 kDa

Gene symbol
PPP1R15A

Synonyms
GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A

Background

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.

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