mouse brain tissue were subjected to SDS PAGE followed by western blot withHK210001 (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP Result of anti-ABL2 (IP:HK210001， 4ug; Detection:HK210001
1:500) with HeLa cells lysate 1200ug.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide usingHK210001 (ABL2 Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HK210001 (ABL2 Antibody) at dilution of 1:50 (under 40x lens).
human liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.