ABL2兔多克隆抗体

 

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210001L ABL2兔多克隆抗体 200ul 2800
KAOKEBIO HK210001M ABL2兔多克隆抗体 100ul 1960

Applications

Tested Applications:
IHC, IP, WB,ELISA


Cited Applications:
WB

mouse brain tissue were subjected to SDS PAGE followed by western blot withHK210001 (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP Result of anti-ABL2 (IP:HK210001, 4ug; Detection:HK210001

1:500) with HeLa cells lysate 1200ug.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide usingHK210001 (ABL2 Antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using HK210001 (ABL2 Antibody) at dilution of 1:50 (under 40x lens).


Species Specificity:
human, mouse, rat

Cited Species:
human, mouse

Positive WB detected in:
mouse brain tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:

human liver cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:
Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information



Full name:
v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)

Calculated molecular weight:
1167 aa, 127 kDa

Observed molecular weight:
128-140 kDa


Gene ID (NCBI):

Gene symbol
ABL2

Synonyms
Abelson related gene protein, ABL2, ABLL, ARG, Tyrosine protein kinase ABL2, Tyrosine protein kinase ARG

Background

ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.

WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
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