ACTA2兔多克隆抗体

 

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210002L ACTA2兔多克隆抗体 200ul 2800
KAOKEBIO HK210002M ACTA2兔多克隆抗体 100ul 1960

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
competitive binding assay, FC, IF, IHC, WB

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue usingHK210002 (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide usingHK210002 (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using HK210002 (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using HK210002 (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

Various lysates were subjected to SDS PAGE followed by western blot withHK210002(smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

IP Result of anti-ACTA2/smooth muscle actin (IP:14395-1-AP, 3ug; Detection:HK210002 1:1000) with mouse liver tissue lysate 6000ug.

1X10^6 C2C12 cell were stained with 0.2ug ACTA2/smooth muscle actin antibody (HK210002 , red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.


Species Specificity:
human, mouse, rat

Cited Species:
human, monkey, mouse, pig, rabbit, rat

Positive WB detected in:
mouse colon tissue, mouse kidney tissue, human placenta tissue, mouse liver tissue

Positive IP detected in:
mouse liver tissue

Positive IHC detected in:

human liver cancer tissue, human heart tissue, stromal tumor tissue, human hysteromyoma tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
C2C12 cell, mouse heart tissue, C2C12 cells

Positive FC detected in:
C2C12 cell

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information



Full name:
actin, alpha 2, smooth muscle, aorta

Calculated molecular weight:
377 aa, 42 kDa

Observed molecular weight:
43 kDa

Gene ID (NCBI):

Gene symbol
ACTA2

Synonyms
a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, Actin, aortic smooth muscle Actin, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, α SMA, α-sma

Background

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:1500-1:6000
IF : 1:20-1:200
咨询客服

联系我们

联系电话:13805711252

咨询客服①:点击这里给我发消息QQ53383090
咨询客服②:点击这里给我发消息QQ16663047

工作时间:周一至周六,8.30-18.00,节假日休息

返回顶部