HSP70兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210178L HSP70兔多克隆抗体 200ul 2800

Applications

Tested Applications:
FC, IF, IHC, IP, WB, ELISA

Cited Applications:
CoIP, IF, IHC, IP, WB

Species Specificity:
human, mouse, rat

Cited Species:
bovine, C. elegans, human, mouse, oyster, pig, rat

Positive WB detected in:
A431 cells, mouse uterus tissue, mouse kidney tissue, rat brain tissue, A549 cells, HeLa cells, Jurkat cells, mouse brain

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:

human breast cancer tissue, human liver cancer tissue, mouse skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HeLa cells, HepG2 cells

Positive FC detected in:
HepG2 cells, HeLa cells, A549 cells

Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
heat shock 70kDa protein 1A

Calculated molecular weight:
70 kDa

Observed molecular weight:
66-70 kDa

Gene symbol
HSPA1A

Synonyms
Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

Background

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)

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