GRP94兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210168S GRP94兔多克隆抗体 50ul 1200

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
WB
Species Specificity:
human, mouse, rat

Cited Species:
human, pig, rat

Positive WB detected in:
HEK-293 cells, A549 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells

Positive IP detected in:
MCF-7 cells

Positive IHC detected in:

human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
MCF-7 cells, HEK-293 cells

Positive FC detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
heat shock protein 90kDa beta (Grp94), member 1

Calculated molecular weight:
96 kDa

Observed molecular weight:
94 kDa

Gene symbol
HSP90B1

Synonyms
ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

Background

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

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