杭州浩克生物
CUX1兔多克隆抗体
6品牌 商品编码 商品名称 规格 单位 单价 HAOKEBIO HK210115L CUX1兔多克隆抗体 200ul 支 2800 HAOKEBIO HK210115S CUX1兔多克隆抗体 50ul 支 1200 Applications Test...
详情咨询GRP94兔多克隆抗体
| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| HAOKEBIO | HK210168S | GRP94兔多克隆抗体 | 50ul | 支 | 1200 |
Applications
Tested Applications:
FC, IF, IHC, IP, WB,ELISA
Cited Applications:
WB
Species Specificity:
human, mouse, rat
Cited Species:
human, pig, rat
Positive WB detected in:
HEK-293 cells, A549 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells, HEK-293 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Product Information
Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Immunogen Information
Full name:
heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight:
96 kDa
Observed molecular weight:
94 kDa
Gene symbol
HSP90B1
Synonyms
ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1
Background
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
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