Cyclin B1兔多克隆抗体

一抗 2420
品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210118L Cyclin B1兔多克隆抗体 200ul 2800

Applications

Tested Applications:
FC, IP, WB, ELISA

Cited Applications:
IF, WB

Species Specificity:
human, mouse

Cited Species:
bovine, human, mouse, rat, sheep

Positive WB detected in:
HeLa cells, PC-3 cells, HepG2 cells, Jurkat cells

Positive IP detected in:
HeLa cells

Positive FC detected in:
Jurkat cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
cyclin B1

Calculated molecular weight:
48 kDa

Observed molecular weight:
55-60 kDa

Gene symbol
CCNB1

Synonyms
G2/mitotic specific cyclin B1

Background

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

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