Caspase 3兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210066L Caspase 3兔多克隆抗体 200ul 2800

Applications

ested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
ELISA, IF, IHC, RIP, WB
Species Specificity:
human, mouse, rat

Cited Species:
Astragalus membranaceus, Bovine, chicken, duck, Goat, hamster, human, monkey, mouse, pig

Positive WB detected in:
Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue

Positive IP detected in:
NIH/3T3 cells

Positive IHC detected in:
mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
NIH/3T3 cells, mouse brain tissue, HeLa cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
caspase 3, apoptosis-related cysteine peptidase

Calculated molecular weight:
32 kDa

Observed molecular weight:
32-35 kDa, 17 kDa, 19 kDa
Gene symbol
CASP3

Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Background

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

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