品牌	商品编码	商品名称	规格	单位	单价
HAOKEBIO	HK510007L	APOL1鼠单克隆抗体	100ul	支	1960

Applications

Tested Applications:

FC, IF, IHC, IP, WB,ELISA

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Cited Applications:

IF, IP, WB

Species Specificity:

human

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Cited Species:

human

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Positive WB detected in:

human plasma tissue

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Positive IP detected in:

human plasma tissue

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Positive IHC detected in:

human liver tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

HepG2 cells

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Positive FC detected in:

HepG2 cells

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Recommended dilution:

WB : 1:20000-1:100000

IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB

IHC : 1:50-1:1000

IF : 1:50-1:500

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG2a

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

apolipoprotein L, 1

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Calculated molecular weight:

44 kDa

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Observed molecular weight:

39-45 kDa

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Gene symbol

APOL1

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Synonyms

APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

Background

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.