CD45兔单克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HKI0461B CD45兔单克隆抗体 1ml 5512.5

Applications

Tested Applications:

FC, IHC, IP, WB,ELISA


Cited Applications:
IF, IHC, WB

Species Specificity:
human, mouse, rat

Cited Species:
Bovine, canine, human, mouse, rat

Positive WB detected in:
mouse thymus tissue, rat thymus tissue, mouse spleen tissue

Positive IP detected in:
mouse spleen tissue

Positive IHC detected in:

human colon tissue, mouse lung tissue, human tonsillitis tissue, human lymphoma tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive FC detected in:
Jurkat cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:1000-1:6000

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
protein tyrosine phosphatase, receptor type, C

Calculated molecular weight:
1304 aa, 147 kDa

Observed molecular weight:
180-220 kDa

Gene symbol
PTPRC

Synonyms
B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

Background

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

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