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IF, IHC, WB,ELISA
human, Canine, mouse, rat
Positive WB detected in:
NCCIT cells, HepG2 cells, EC109 cells, K-562 cells, HSCT6 cells, NIH3T3 cells
Positive IHC detected in:
human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MDCK cells, HeLa cells
WB : 1:2000-1:12000
IHC : 1:200-1:1000
IF : 1:50-1:500
Protein A purification
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
tubulin, gamma 1
Calculated molecular weight:
Observed molecular weight:
Gamma 1 tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin gamma 1 chain, tubulin, gamma 1, tubulin-gamma
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.