Caspase 6兔多克隆抗体

一抗 3130
品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210068L Caspase 6兔多克隆抗体 200ul 2800

Applications

Tested Applications:
IHC, WB,ELISA

Cited Applications:
WB
Species Specificity:
human, mouse, rat

Cited Species:
human, rat

Positive WB detected in:
Staurosporine treated Jurkat cells, mouse brain tissue, Jurkat cells

Positive IHC detected in:
human prostate cancer tissue, human lung cancer tissue, human skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
caspase 6, apoptosis-related cysteine peptidase

Calculated molecular weight:
33 kDa, 22 kDa

Observed molecular weight:
33-35 kDa

Gene symbol
CASP6

Synonyms
Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase 6/p18/p11, Caspase6, MCH2

Background

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

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