PCNA鼠单克隆抗体

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PCNA鼠单克隆抗体

PCNA鼠单克隆抗体

  • 价格¥2800
  • 规格200ul
  • 货号HK510145L
  • 单位
  • 品牌HaoKebio

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HAOKEBIOHK510145LPCNA鼠单克隆抗体200ul2800

Applications

Tested Applications:

FC, IF, IHC, IP, WB, ELISA


Cited Applications:

IF, IHC, WB


Species Specificity:

human, mouse, pig, rat


Cited Species:

human, mouse, pig, rabbit, rat


Positive WB detected in:

HEK-293 cells, HeLa cells, HepG2 cells


Positive IP detected in:

HepG2 cells


Positive IHC detected in:

human gliomas tissue, human tonsillitis tissue, human breast cancer tissue, mouse ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:

HepG2 cells, mouse heart tissue


Positive FC detected in:

MCF-7 cells


Recommended dilution:

WB : 1:5000-1:50000

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

IHC : 1:500-1:2000

IF : 1:250-1:1000

Product Information


Source:

Mouse


Purification method:

Protein A purification


Isotype:

IgG1


Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:

proliferating cell nuclear antigen


Calculated molecular weight:

29 kDa/31 kDa


Observed molecular weight:

36-38 kDa


Gene symbol

PCNA


Synonyms

Cyclin, PCNA

Background

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

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