IFT20兔多克隆抗体

一抗 2400
品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210185L IFT20兔多克隆抗体 200ul 2800
HAOKEBIO HK210185S IFT20兔多克隆抗体 50ul 1200

Applications

Tested Applications:
IF, IHC, IP, WB, ELISA

Cited Applications:
CoIP, FC, IF, IHC, IP, WB

Species Specificity:
human, mouse, Canine, rat

Cited Species:
canine, human, mouse, rat, zebrafish

Positive WB detected in:
HEK-293 cells, mouse testis tissue, MDCK cells, rat testis

Positive IP detected in:
mouse testis tissue

Positive IHC detected in:

human endometrial cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
hTERT-RPE1 cells, MDCK cells

Recommended dilution:
WB : 1:300-1:800
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
intraflagellar transport 20 homolog (Chlamydomonas)

Calculated molecular weight:
15 kDa

Observed molecular weight:
15-18 kDa

Gene symbol
IFT20

Synonyms
hIFT20, IFT20

Background

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

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