CD34兔多克隆抗体

一抗 4140
品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210081L CD34兔多克隆抗体 200ul 2800
HAOKEBIO HK210081M CD34兔多克隆抗体 100ul 1960

Applications

Tested Applications:
IF, IHC, WB,ELISA

Cited Applications:
FC, IF, IHC, WB
Species Specificity:
human

Cited Species:
bovine, human, rabbit

Positive WB detected in:
HL-60 cells

Positive IHC detected in:

human tonsillitis tissue, human small intestine tissue, human gliomas tissue, human hysteromyoma tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
human tonsillitis tissue, human liver cancer tissue

Recommended dilution:
WB : 1:500-1:1000
IHC : 1:300-1:1200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
CD34 molecule

Calculated molecular weight:
41 kDa

Observed molecular weight:
105-120 kDa

Gene symbol
CD34

Synonyms
CD34, CD34 molecule

Background

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.

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