BIP兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210047L BIP兔多克隆抗体 200ul 2800
KAOKEBIO HK210047S BIP兔多克隆抗体 50ul 1200

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
CoIP, IF, IHC, IP, WB

Species Specificity:
human, mouse, rat

Cited Species:
bovine, carp, chicken, deer, fish, Hamster, human, mouse, pig, rat

Positive WB detected in:
HEK-293 cells

Positive IP detected in:
MCF-7 cells

Positive IHC detected in:
mouse kidney tissue, human breast cancer tissue, human pancreas cancer tissue, mouse heart tissue, rat brain tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HepG2 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)

Calculated molecular weight:
78 kDa

Observed molecular weight:
70-78 kDa

Gene symbol
HSPA5

Synonyms
BIP, GRP 78, GRP78, HSP A5, HSPA5, MIF2

Background

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

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