ERO1L兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210137S ERO1L兔多克隆抗体 50ul 1200

Applications

Tested Applications:
FC, IHC, WB,ELISA

Cited Applications:
WB
Species Specificity:
human, mouse, rat

Cited Species:
mouse, pig

Positive WB detected in:
mouse ovary tissue, A431 cells

Positive IHC detected in:
human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ERO1-like (S. cerevisiae)

Calculated molecular weight:
468 aa, 54 kDa

Observed molecular weight:
54 kDa

Gene symbol
ERO1L

Synonyms
ERO1 alpha, ERO1 L, ERO1 L alpha, ERO1 like (S. cerevisiae), ERO1 like protein alpha, ERO1L, Oxidoreductin 1 L alpha

Background

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. This antibody has no cross reaction to ERO1.

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