ARF4兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210014L ARF4兔多克隆抗体 200ul 2800
KAOKEBIO HK210014M ARF4兔多克隆抗体 100ul 1960

Applications

 

Tested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
IF, IHC, WB

Species Specificity:
human, mouse, rat

Cited Species:
human, mouse

Positive WB detected in:
HT-29 cells, rat brain tissue, mouse brain tissue, K-562 cells, HepG2 cells, HeLa cells, HT-29 cells

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HepG2 cells

Positive FC detected in:
K-562 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ADP-ribosylation factor 4

Calculated molecular weight:
180 aa, 21 kDa

Observed molecular weight:
21 kDa

Gene ID (NCBI):

Gene symbol
ARF4

Synonyms
ADP ribosylation factor 4, ARF2, ARF4

Background

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

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