品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HK510016L	BAX鼠单克隆抗体	200ul	支	2800

Applications

Tested Applications:

FC, IHC, WB,ELISA

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Cited Applications:

CoIP, IF, IHC, WB

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Species Specificity:

human, mouse, rat

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Cited Species:

human, mouse, pig, rat

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Positive WB detected in:

HeLa cells, human testis tissue, HEK-293 cells, COLO 320 cells, PC-12 cells, ROS1728 cells, Neuro-2a cells

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Positive IHC detected in:

human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive FC detected in:

MCF-7 cells

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Recommended dilution:

WB : 1:5000-1:20000

IHC : 1:500-1:2000

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG2b

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

BCL2-associated X protein

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Calculated molecular weight:

21 kDa

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Observed molecular weight:

21 kDa

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Gene symbol

BAX

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Synonyms

Apoptosis regulator BAX, BAX

Background

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).