品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HK510172S	TNF alpha鼠单克隆抗体	50ul	支	1200

Applications

Tested Applications:

IF, IHC, WB, ELISA

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Cited Applications:

ELISA, IF, IHC, WB

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Species Specificity:

human, mouse, rat

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Cited Species:

chicken, human, monkey, mouse, rat

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Positive WB detected in:

U-937 cells, HeLa cells, THP-1 cells, 4T1 cells, HL-60 cells, PC-12 cells, HSC-T6 cells

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Positive IHC detected in:

human breast cancer tissue, human liver cancer tissue, human colon cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

human breast cancer tissue

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Recommended dilution:

WB : 1:1000-1:8000

IHC : 1:200-1:1000

IF : 1:50-1:500

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG2b

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

tumor necrosis factor (TNF superfamily, member 2)

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Calculated molecular weight:

233 aa, 26 kDa

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Observed molecular weight:

17-22 kDa

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Gene symbol

TNF

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Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.