p40兔单克隆抗体

一抗 产品 抗体 2890
p40兔单克隆抗体

p40兔单克隆抗体

  • 价格¥2250
  • 规格100ul
  • 货号HKI0171A
  • 单位
  • 品牌HaoKebio

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HaoKebioHKI0171Ap40兔单克隆抗体100ul2250

Applications

Tested Applications:

IF, IHC, WB,ELISA


Species Specificity:

Human, mouse, rat


Positive WB detected in:

Jurkat cells, HeLa cells, HEK-293 cells, HSCT6 cells, RAW 264.7 cells


Positive IHC detected in:

human lung cancer tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:

A549 cells


Recommended dilution:

WB : 1:1000-1:6000

IHC : 1:500-1:2000

IF : 1:50-1:500

Product Information


Source:

Mouse


Purification method:

Protein A purification


Isotype:

IgG1


Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:

Rab9 effector protein with kelch motifs


Calculated molecular weight:

41 kDa


Observed molecular weight:

40 kDa


Gene symbol

RABEPK


Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK

Background

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network

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