c-Met (Cytoplasmic)兔多克隆抗体

一抗 未分类 3500
品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210105L c-Met (Cytoplasmic)兔多克隆抗体 200ul 2800

Applications

Tested Applications:
IHC, IP, WB,ELISA

Cited Applications:
IF, IHC, IP, WB
Species Specificity:
human, canine, mouse, rat

Cited Species:
human, mouse, rat

Positive WB detected in:
mouse liver tissue, MDCK cells, HepG2 cells, A431 cells, rat liver tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue, human colon tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
met proto-oncogene (hepatocyte growth factor receptor)

Calculated molecular weight:
1390 aa, 155 kDa

Observed molecular weight:
145 kDa

Gene symbol
MET

Synonyms
AUTS9, c-Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

Background

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

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