Beclin 1兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210044L Beclin 1兔多克隆抗体 200ul 2800

Applications

Tested Applications:
IF, IHC, IP, WB,ELISA

Cited Applications:
CoIP, IF, IHC, IP, WB
Species Specificity:
human, mouse, rat

Cited Species:
Bovine, chicken, duck, fish, hamster, human, mouse, pig, prawn, rat

Positive WB detected in:
C6 cells, L02 cells, HEK-293 cells, A431 cells, PC-3 cells, HT-1080 cells, human placenta tissue, HeLa cells, NIH/3T3 cells, Raji cells, mouse brain tissue, rat brain tissue

Positive IP detected in:
MDA-MB-453s cells

Positive IHC detected in:
human breast cancer tissue, human breast hyperplasia tissue, human prostate hyperplasia tissue, human cervical cancer tissue, human colon cancer tissue, human stomach tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
NIH/3T3 cells, mouse heart tissue

Recommended dilution:
WB : 1:1000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
beclin 1, autophagy related

Calculated molecular weight:
52 kDa

Observed molecular weight:
52 kDa

Gene symbol
BECN1

Synonyms
ATG6, Beclin 1, beclin 1, autophagy related, beclin1, BECN1, GT197, Protein GT197, VPS30

Background

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

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