杭州浩克生物
AMBRA1兔多克隆抗体
147Applications Product Information Immunogen Information Background
查看全文ATG14兔多克隆抗体
| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| KAOKEBIO | HK210022L | ATG14兔多克隆抗体 | 200ul | 支 | 2800 |
Application
Tested Applications:
IHC, IP, WB,ELISA
Species Specificity:
Human, Mouse, Rat
Positive WB detected in:
mouse brain tissue, A549 cells, rat brain tissue
Positive IP detected in:
rat brain tissue
Positive IHC detected in:
human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:200-1:800
Product Information
Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Immunogen Information
Full name:
KIAA0831
Calculated molecular weight:
55 kDa
Observed molecular weight:
55 and 65 kDa
Gene symbol
KIAA0831
Synonyms
ATG14, Atg14L, Barkor, KIAA0831
Background
ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)
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