ATG14兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210022L ATG14兔多克隆抗体 200ul 2800

Application

Tested Applications:
IHC, IP, WB,ELISA
Species Specificity:
Human, Mouse, Rat

Positive WB detected in:
mouse brain tissue, A549 cells, rat brain tissue

Positive IP detected in:
rat brain tissue

Positive IHC detected in:

human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:200-1:800

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
KIAA0831

Calculated molecular weight:
55 kDa

Observed molecular weight:
55 and 65 kDa

Gene symbol
KIAA0831

Synonyms
ATG14, Atg14L, Barkor, KIAA0831

Background

ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)

 

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