ATG12兔多克隆抗体

一抗 3240
品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210020L ATG12兔多克隆抗体 200ul 2800

Applications

Tested Applications:
IHC, IP, WB, ELISA
Cited Applications:
IHC, WB
Species Specificity:
human, mouse

Cited Species:
human, mouse, rat

Positive WB detected in:
human bladder tissue, UV treated HEK-293

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ATG12 autophagy related 12 homolog (S. cerevisiae)

Calculated molecular weight:
16 kDa

Observed molecular weight:
48-50 kDa

Gene symbol
ATG12

Synonyms
APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12

Background

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

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