APAF1兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
KAOKEBIO HK210012L APAF1兔多克隆抗体 200ul 2800
KAOKEBIO HK210012S APAF1兔多克隆抗体 50ul 1200

Applications

Tested Applications:
IF, IHC, IP, WB,ELISA

Cited Applications:
IF, IHC, WB
Species Specificity:
human, rat

Cited Species:
human, mouse, rat

Positive WB detected in:
HEK-293 cells, A549 cells, COLO 320 cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
A549 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
apoptotic peptidase activating factor 1

Calculated molecular weight:
1248 aa, 142 kDa

Observed molecular weight:
130-142 kDa

Gene symbol
APAF1

Synonyms
APAF 1, APAF1, CED4, KIAA0413

Background

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

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