品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKI0171A	p40兔单克隆抗体	100ul	支	2250

Applications

Tested Applications:

IF, IHC, WB,ELISA

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Species Specificity:

Human, mouse, rat

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Positive WB detected in:

Jurkat cells, HeLa cells, HEK-293 cells, HSCT6 cells, RAW 264.7 cells

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Positive IHC detected in:

human lung cancer tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

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Positive IF detected in:

A549 cells

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Recommended dilution:

WB : 1:1000-1:6000

IHC : 1:500-1:2000

IF : 1:50-1:500

Product Information

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Source:

Mouse

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Purification method:

Protein A purification

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Isotype:

IgG1

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Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

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Full name:

Rab9 effector protein with kelch motifs

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Calculated molecular weight:

41 kDa

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Observed molecular weight:

40 kDa

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Gene symbol

RABEPK

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Synonyms

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK

Background

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network