PLA2R Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150307 Clone# BP6284 Predicted Molecular Wt: 169kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q13018 Background: M-type phospholipase A2 receptor(PLA2R) , acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2- IIA/PLA2G2A. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis. PLA2R is highly expressed in podocytes in human glomeruli, also expressed in pancreas, amnion, choriodecidua and placenta. PLA2R was identified as a major target antigen involved in idiopathic membranous nephropathy in adults. Circulating autoantibodies against PLA2R were found in 70 to 80% of patients with idiopathic membranous nephropathy . It has been suggested that the serum level of PLA2R autoantibody could be used for the diagnosis of idiopathic membranous nephropathy and for the monitoring of response to treatment. Subcellular location: Membranous Recommended Method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide. This information is proprietary to Biolynx. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to – 18°C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. Beck LH Jr, et al. Kidney Int 77:765-770, 2010. 2. R.J. Glassock.Contrib Nephrol, 181:131-142,2013. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glomeruli tissue sections labeling PLA2R with BP6284.
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