NapsinA兔单克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HKI0661B NapsinA兔单克隆抗体 1ml 10106.25

Applications

Tested Applications:
IF, IHC, WB,ELISA

Species Specificity:
human, mouse

Positive WB detected in:
mouse lung tissue

Positive IHC detected in:

human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
PC-3 cells

Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
napsin A aspartic peptidase

Calculated molecular weight:
420 aa, 45 kDa

Observed molecular weight:
45-55 kDa

Gene symbol
NAPSA

Synonyms
Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02

Background

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

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