GranzymeB兔单克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HKI0291B GranzymeB兔单克隆抗体 1ml 10500

Applications

Tested Applications:
FC, IF, IHC, WB,ELISA

Cited Applications:
IF, IHC, WB


Species Specificity:
human

Cited Species:
human, mouse

Positive WB detected in:
HL-60 cells

Positive IHC detected in:

human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HeLa cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

Calculated molecular weight:
247 aa, 28 kDa

Observed molecular weight:
33 kDa

Gene symbol
GZMB

Synonyms
CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, Granzyme B, GZMB, HLP, SECT

Background

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and g high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751).

Protocols

咨询客服

联系我们

联系电话:13805711252

咨询客服①:点击这里给我发消息QQ53383090
咨询客服②:点击这里给我发消息QQ16663047

工作时间:周一至周六,8.30-18.00,节假日休息

返回顶部