PD-1兔单克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HKI0671A PD-1兔单克隆抗体 100ul 1500

Applications

Tested Applications:
FC, IF, IHC, WB,ELISA
Cited Applications:
IF, IHC, WB

Species Specificity:
human, rat, mouse

Cited Species:
human, mouse, rat

Positive WB detected in:
mouse thymus tissue, human lymph node tissue, rat spleen tissue

Positive IHC detected in:

human tonsillitis tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
human tonsillitis tissue

Positive FC detected in:
MOLT-4 cells

Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:2000-1:8000
IF : 1:200-1:800

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
programmed cell death 1

Calculated molecular weight:
288 aa, 32 kDa

Observed molecular weight:
32 kDa, 47-55 kDa

Gene symbol
PDCD1

Synonyms
CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

Background

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438)

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