PCNA鼠单克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK510145L PCNA鼠单克隆抗体 200ul 2800

Applications

Tested Applications:
FC, IF, IHC, IP, WB, ELISA

Cited Applications:
IF, IHC, WB

Species Specificity:
human, mouse, pig, rat

Cited Species:
human, mouse, pig, rabbit, rat

Positive WB detected in:
HEK-293 cells, HeLa cells, HepG2 cells

Positive IP detected in:
HepG2 cells

Positive IHC detected in:

human gliomas tissue, human tonsillitis tissue, human breast cancer tissue, mouse ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HepG2 cells, mouse heart tissue

Positive FC detected in:
MCF-7 cells

Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:500-1:2000
IF : 1:250-1:1000

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
proliferating cell nuclear antigen

Calculated molecular weight:
29 kDa/31 kDa

Observed molecular weight:
36-38 kDa

Gene symbol
PCNA

Synonyms
Cyclin, PCNA

Background

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

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