TLR4兔多克隆抗体

一抗 90
品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210332M TLR4兔多克隆抗体 100ul 1960

Applications

Tested Applications:
IHC, WB,ELISA

Cited Applications:
CoIP, ELISA, IF, IHC, IP, Microtiter plate binding assay, WB

Species Specificity:
human, mouse, rat

Cited Species:
bovine, hamster, human, mouse, pig, rat

Positive WB detected in:
mouse liver tissue, Daudi cells, rat liver tissue

Positive IHC detected in:

human placenta tissue, human liver cancer tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
toll-like receptor 4

Calculated molecular weight:
839 aa, 96 kDa

Observed molecular weight:
90-100 kDa

Gene symbol
TLR4

Synonyms
ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4

Background

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. The calculated molecular weights of the three TLR4 isoforms are 96, 91, and 73 kDa.

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