PEX14兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210272S PEX14兔多克隆抗体 50ul 1200

Applications

Tested Applications:
FC, IF, IHC, IP, WB, ELISA

Cited Applications:
IF, IHC, WB
Species Specificity:
human, mouse, rat, monkey

Cited Species:
human, monkey, mouse, rat

Positive WB detected in:
A431 cells, HEK-293T cells, HeLa cells, SKOV-3 cells

Positive IP detected in:
mouse liver tissue

Positive IHC detected in:

human liver cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:500-1:2000

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
peroxisomal biogenesis factor 14

Calculated molecular weight:
41 kDa

Observed molecular weight:
57 kDa

Gene symbol
PEX14

Synonyms
NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein

Background

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

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