PAX8兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210272S PAX8兔多克隆抗体 50ul 1200

Applications

Tested Applications:
IF, IHC, WB,ELISA

Cited Applications:
FC, IF, IHC, WB
Species Specificity:
human, mouse, rat

Cited Species:
bovine, human, mouse, pig, rat, zebrafish

Positive WB detected in:
SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells

Positive IHC detected in:

human ovary tumor tissue, human thyroid cancer tissue, human renal cell carcinoma tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
SKOV-3 cells, mouse kidney tissue

Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:1000-1:5000
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
paired box 8

Calculated molecular weight:
48 kDa

Observed molecular weight:
48 kDa, 58 kDa

GenBank accession number:

Gene symbol
PAX8

Synonyms
paired box 8, Paired box protein Pax 8, PAX8

Background

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

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