NRF2兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210254M NRF2兔多克隆抗体 100ul 1960

Applications

Tested Applications:
FC, IF, IHC, IP, WB, ELISA

Cited Applications:
chIP, CoIP, IF, IHC, IP, WB
Species Specificity:
human, mouse, rat

Cited Species:
Bovine, chicken, Codonopsis lanceolata, ducks, hamster, human, monkey, mouse, pig, rabbit

Positive WB detected in:
HeLa cells, DMSO treated HeLa cells, rat liver tissue

Positive IP detected in:
mouse kidney tissue

Positive IHC detected in:

human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human liver cancer tissue, human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HepG2 cells

Positive FC detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
nuclear factor (erythroid-derived 2)-like 2

Calculated molecular weight:
605 aa, 68 kDa

Observed molecular weight:
110 kDa, 68 kDa

GenBank accession number:

Gene symbol
NFE2L2

Synonyms
HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2

Background

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the biologically relevant molecular weight of Nrf2 is ~95-110 kDa and not the predicted ~55-65 kDa based on its 2-kilobase open reading frame. The data discussed and presented here will hopefully lead to a uniform acceptance that future experiments and publications should be designed around detecting Nrf2 at the correct molecular weight of ~95-120 kDa (PMID: 22703241).

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