KI67兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210197L KI67兔多克隆抗体 200ul 2800

Applications

Tested Applications:

FC, IF, IHC,ELISA


Cited Applications:
IF, IHC
Species Specificity:
human

Cited Species:
hamster, human, pig

Positive IHC detected in:

human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human lymphoma tissue, human skin cancer tissue, Insulinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
HeLa cells

Positive FC detected in:
Jurkat cells

Recommended dilution:
IHC : 1:2000-1:10000
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
antigen identified by monoclonal antibody Ki-67

Calculated molecular weight:
359 kDa

Observed molecular weight:

Gene symbol
MKI67

Synonyms
Antigen KI 67, Ki 67, KI67, KIA, MKI67

Background

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

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