LC3兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210204L LC3兔多克隆抗体 200ul 2800
HAOKEBIO HK210204S LC3兔多克隆抗体 50ul 1200

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA

Cited Applications:
IF, IHC, IP, WB

Species Specificity:
human, mouse, rat

Cited Species:
bovine, duck, goat, human, monkey, mouse, pig, rabbit, rat, Rhesus

Positive WB detected in:
Chloroquine treated HepG2 cells, HEK-293 cells, Chloroquine treated HEK-293 cells, HeLa cells, HSC-T6 cells, human skeletal muscle tissue, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, NIH/3T3 cells, rat brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:

human gliomas tissue, mouse brain tissue, human testis tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, HepG2 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:300-1:1200
IF : 1:100-1:400

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
microtubule-associated protein 1 light chain 3 beta

Calculated molecular weight:
15 kDa

Observed molecular weight:
14-18 kDa

Gene symbol
MAP1LC3B

Synonyms
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B

Background

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

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