杭州浩克生物
HSP70兔多克隆抗体
171Applications Product Information Immunogen Information Background
查看全文LC3兔多克隆抗体
| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| HAOKEBIO | HK210204L | LC3兔多克隆抗体 | 200ul | 支 | 2800 |
| HAOKEBIO | HK210204S | LC3兔多克隆抗体 | 50ul | 支 | 1200 |
Applications
Tested Applications:
FC, IF, IHC, IP, WB,ELISA
Cited Applications:
IF, IHC, IP, WB
Species Specificity:
human, mouse, rat
Cited Species:
bovine, duck, goat, human, monkey, mouse, pig, rabbit, rat, Rhesus
Positive WB detected in:
Chloroquine treated HepG2 cells, HEK-293 cells, Chloroquine treated HEK-293 cells, HeLa cells, HSC-T6 cells, human skeletal muscle tissue, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse testis tissue, NIH/3T3 cells, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human gliomas tissue, mouse brain tissue, human testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:300-1:1200
IF : 1:100-1:400
Product Information
Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Immunogen Information
Full name:
microtubule-associated protein 1 light chain 3 beta
Calculated molecular weight:
15 kDa
Observed molecular weight:
14-18 kDa
Gene symbol
MAP1LC3B
Synonyms
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B
Background
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
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