IFT140兔多克隆抗体

一抗 80
品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210184L IFT140兔多克隆抗体 200ul 2800

Applications

Tested Applications:
IF, IHC, IP, WB,ELISA

Cited Applications:
CoIP, IF, IHC, WB
Species Specificity:
human, mouse, rat

Cited Species:
canine, human, mouse, zebrafish

Positive WB detected in:
mouse testis tissue, HepG2 cells, rat testis tissue

Positive IP detected in:
rat testis tissue

Positive IHC detected in:

mouse testis tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
mouse testis tissue

Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:200-1:800

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
intraflagellar transport 140 homolog (Chlamydomonas)

Calculated molecular weight:
1462 aa, 165 kDa

Observed molecular weight:
140 kDa

Gene symbol
IFT140

Synonyms
c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2

Background

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

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