杭州浩克生物
E-Cadherin兔单克隆抗体
163Tested Applications: FC, IF, IHC, WB,ELISA Cited Applications: FC, IF, IHC, WB Species Specificity...
查看全文IFT140兔多克隆抗体
| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| HAOKEBIO | HK210184L | IFT140兔多克隆抗体 | 200ul | 支 | 2800 |
Applications
Tested Applications:
IF, IHC, IP, WB,ELISA
Cited Applications:
CoIP, IF, IHC, WB
Species Specificity:
human, mouse, rat
Cited Species:
canine, human, mouse, zebrafish
Positive WB detected in:
mouse testis tissue, HepG2 cells, rat testis tissue
Positive IP detected in:
rat testis tissue
Positive IHC detected in:
mouse testis tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse testis tissue
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:200-1:800
Product Information
Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Immunogen Information
Full name:
intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight:
1462 aa, 165 kDa
Observed molecular weight:
140 kDa
Gene symbol
IFT140
Synonyms
c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2
Background
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
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