ICAM-1兔多克隆抗体

品牌 商品编码 商品名称 规格 单位 单价
HAOKEBIO HK210179L ICAM-1兔多克隆抗体 200ul 2800

Applications

Tested Applications:
FC, IF, IHC, IP, WB, ELISA

Cited Applications:
Blocking, IF, IHC, IP, WB
Species Specificity:
human

Cited Species:
bovine, human

Positive WB detected in:
HUVEC cells, Raji cells, L02 cells

Positive IP detected in:
Raji cells

Positive IHC detected in:

human lung cancer tissue, human tonsillitis tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Positive IF detected in:
human lung cancer tissue

Positive FC detected in:
Raji cells

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
intercellular adhesion molecule 1

Calculated molecular weight:
90 kDa

Observed molecular weight:
85-95 kDa

Gene symbol
ICAM1

Synonyms
BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

Background

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:200-1:800
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